Incidence of bean anthracnose in Western Kenya and its management using aqueous extract of Aloe vera
##article.abstract##
High seed exchange and frequent resistance breakdown continue to be a challenge in managing seed borne fungi Colletotrichum lindemuthianum in Western Kenya farm saved bean seeds. Continuous pathogen surveillance can enable timely development and application of effective environment friendly mitigation strategies. The objective of this study was to determine the presence C. lindemuthianum on farm-saved bean seeds collected from seven different regions of Western Kenya and its possible control using aqueous plant extracts at different concentration in comparison to traditional fungicides. Systematic random sampling method was used to collect 196 farms saved bean seeds from farmers in the agro-ecological zones within the regions for four seasons between 2008 and 2010. Incidence was scored for each sampled seed lot. Water extract of Aloe vera was screened in vitro for antifungal activities against C. lindemuthianum using poison food technique. Both incidence and inhibitory data were subjected to analysis of variance using GenStat® computer package. The sampled eight seed varieties namely Red haricot (GLP- 585), Mwitemania GLP X92, Yellow (KAT B 1), Rose coco (GLP-288), White, Rose coco (GLP-2), Brown and Mwezi moja (GLP-1004) had high and significantly difference (p≤0.05) anthracnose incidences. Variety GLP 585 had the highest incidence (53.1%) whereas GLP 2 had the lowest (28.7%). Aqueous extract from Aloe vera plant exhibited a higher antifungal activity at all concentration levels (30, 25 and 20%) tested and compared well with the standard synthetic fungicide; Mancozeb. There is high disease incidence in the farm saved seeds in Western Kenya and this could be due to high seed exchange and the warm, humid environment. However, farmers should be encouraged to use environment friendly, affordable and effective aqueous plant extract of Aloe vera.
References
Allen, D. J, Buruchara, R. A and Smithson, J. B., (1998). Diseases of Common Bean. In. D. J. Allen and J. M. Lenne (CIAT editors), (1998). The pathology of Food and Pasture Legumes. CAB International, PP 179-265
Barrus, M. F., (1911). Variation of varieties of beans in their susceptibility to anthracnose. Phytopathology 1: 190-195.
Begum, F. and Bhuiyan M. K. A., (2006). Integrated control of seedling mortality of lentil caused by Sclerotum rolfsii. Bangladesh Journal of Plant Panthology 23: 60-65.
Bruneton, J., (1993). Pharmacognosie. Phytochimie. Plantes médicinales, Tec Doc, Paris p. 363.
Buruchara, R., (2007). Background information on Common Beans (Phaseolus vulgaris L) in Biotechnology, Breeding & Seed Systems for African Crops. http://www.africancrops.net/rockefeller/crops/beans/index.htm.
FAO, (Food and Agricultural Organization), (2007). FAO Statistics (FAOSTAT). http://faostat.fao.org/site/567/Desktop. Default.aspx?PageID=567. (Accessed 12 April 2009).
FAO, (Food and Agricultural Organization), (2008). Food Security Statistics, 1995 – 2003
FAOSTAT (Food and Agricultural Organization Statistics), (2013). crop production http://faostat.fao.org/site/567/default.aspx#ancor).
FAOSTAT, (Food and Agricultural Organization Statistics), (2008). Food Security Statistics. www.fao.org 1995 – 2003
Fernandez, M. T., Fernandez M., Casares A., Rodriguez R., Fueyo M., (2000). Bean germplasm evaluation for anthracnose resistance and characterization of agronomic traits: A new physiological strain of Colletotrichum lindemuthianum infecting Phaseolus vulgaris L. in Spain. Euphytica 114: 143-149.
ISTA, (International Seed Testing Association), (2008). Detection of Colletotrichum lindemuthianum on PhaseoIus vulgaris (Bean). Annex to Chapter 7: Seed Health Methods 7-006-5.
Jaya, G. and Dhananjay T., (2012) Antifungal potential of crude plant extract on some pathogenic fungi. www.worldjournalofscience.com. World Journal of Science and Technology, 2(6):58-62.
Jerba, V. F., Rodella R. A. and Furtado E. l., (2005). Relationship between leaf structure and Glomerella cingulata f.sp. phaseolus preinfection Pesqui. Agropecu. Bras. 217-223.
Masangwa, J. I. G., Aveling T. A. S. and Kritzinger Q., (2012). Screening of plant extracts for antifungal activities against Colletotrichum species of common bean (Phaseolus vulgaris L.) and cowpea (Vigna unguiculata (L.) Walp) Journal of Agricultural Science, Page 1 of 10
Masoko, P., Picard J. and Eloff J. N., (2005). Antifungal activities of six South African Terminalia species (Combretaceae). Journal of Ethnopharmacology 99: 301 – 308
Nkalubo, S., (2007). Study of anthracnose (Colletotrichum lindemuthianum) resistance and its inheritance in Ugandan dry bean germplasm: A thesis submitted to the
Faculty of Science and Agriculture, University of Kwazulu-Natal Pietermantzburg, South Africa. African Crop Science Society 8 : 869-874.
Ombiri, J., Zinkernagel, V., Gathura E.M., Achwanya, O. and Lebeda, A., (2003). Induction of post-infection ethylene and its role in resistance of bean (Phaseolus vulgaris) against Colletotrichum lindemuthianum. Plant protection science, 39: 79-87.
Opole, R. A., Mathenge P. W., Auma E. O., Van Rheenen H. A., Almekinders C. J. M., (2003). On-farm seed production practices of common bean (Phaseolus vulgaris L.) African Crop Science Conference Proceedings, Vol. 6:722-725.
Satish, S., Mohana D. C., Ranhavendra M. P. and Raveesha K. A., (2007). Antifungal activity of some plant extracts against important seed borne pathogen of Aspergillus sp. Journal of Agricultural Technology 3(1): 109-119.
Sharma P. N., Partial S., Sharma O. P. and Renu K., (2004). Yield loss assessment in Kidney bean due to bean anthracnose (Colletotrichum lindemuthianum) under sub-temperate conditions of Himachal Pradesh. : National Symposium on Emerging Trends in Disease Management, 9-10, CSK HPKV, Palampur, p. 19. (Abstr.)
Shovan, L. R., Bhuiyan, M. K. A., Begum, J.A. and Pervez, Z., (2008). In vitro control of Colletotrichum dematium causing anthracnose of soybean by fungicides, plant extracts and Trichoderma harzianum. International Journal of Sustainable Crop Production 3: 10–17.
Sing, J. and Tripathi, N. N., (1999). Inhibition of storage fungi of blackgram (Vigna mungo) by some essential oils. Flavour and Fragrance Journal 14: 1-4.
Tegegne, G., Pretorius, J. C. & Swart, W. J., (2008). Antifungal properties of Agapanthus africanus L. extracts gainst plant pathogens. Crop Protection 27: 1052–1060.
Tesfaye, B. M., (2003). Biology and Control of Bean Anthracnose in Ethiopia. A PhD Thesis submitted to the Faculty of Natural and Agricultural Sciences, University of Free State. Bloemfontein, South Africa.
Tu, J. C., (1992). Colletotrichum lindemuthianum on bean. Population dynamics of the pathogen and breeding for resistance. Pages 203-224 in: Colletotrichum: Biology, Pathology and Control. J. A. Bailey and M. J. Jeger, eds. CAB International, Wallingford, UK.
Uzma S., Nusrat H., Jawed N., (2011). Antifungal activity of Aloe vera gel against plant pathogenic fungi . Pakistan Journal Botany. 43(4): 2231-2233.
Vsn International (VSNi), (2008). GenStat Discovery, edition 3. Software (now ed. 4) available online at http://www. vsni.co.uk (verified 3 May 2012).
Wokocha, R. C. and Okereke V. C., (2005). Fungitoxic activity of extracts of some Medicinal plants on Sclerotium rolfsii, causal organism of the Basal Stem Rot Diseases of Tomato. Niger. Journal of Plant Protection. 22: 106- 110.
www.mapsofworld.com
Yesuf, M. and Sangchote S., (2007). Survival and tansmission of Colletotrichum lindemuthianum fom naturally infected common bean seeds to the seedlings. Tropical Science. 47 (2): 96-103.
Zaumeyer, W.j. and Meiners J.P., (1975) Disesse resistance in beans. Annual review of phytopathology, 13:313-334
